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CDM-HD Serum Replacement

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CDM-HD Serum Replacement

CHEMICALLY DEFINED MEDIUM FOR
HIGH DENSITY CELL CULTURE

CDM-HD serum replacement optimized for Hollow Fiber Bioreactors

CDM-HD is a chemically defined, protein free, animal component free cGMP compliant serum replacement optimized for use in hollow fiber bioreactors.

The culture of cells at in-vivo like cell densities allows cells to generate their own micro-environment and reduces dependency on exogenous factors found in serum or serum-free mediums. However, commercial serum free mediums can be expensive, contain surfactants, and are each optimal for only a limited number of cell types.

The concept of CDM-HD is simple: produce a universal serum substitute that can take advantage of the simplified serum requirements for cells grown at high densities in hollow fiber bioreactor systems. CDM-HD represents a true breakthrough in cell culture ideology and technology. It represents the first time that a cell culture medium can be simplified and optimized for the unique hollow fiber bioreactor bio-environment.

Data courtesy of Dr. Erin Bromage c/o the US Veterinary Immune Reagents Network

Above: Unpurified antibody harvest from a C2011 hollow fiber bioreactor. The antibody of interest is the predominant protein present.

Secreted products such as monoclonal antibodies and recombinant proteins are free of contaminating proteins from the medium and can be purified using simplified protocols, increasing net yield in many cases. CDM-HD provides lot-to-lot consistency and is an economical replacement for serum. It is available as a dry powder to make up one liter and is used at a concentration of 10%. It can be shipped at ambient temperature and has a three-year shelf life (from date of manufacture) when stored at 4 degrees. CDM-HD is cost effective, giving you all the benefits of a chemically defined, protein-free medium without the high price.

FEATURES
BENEFITS
Chemically defined Lot-to-lot consistency
Protein free Purification of secreted products simplified
Animal component free Regulatory compliance simplified, no agents of unknown origin, mycoplasma, viruses
Dry powder Long shelf life
Store at 4 degrees Simple storage conditions

Optimized for Hollow Fiber Bioreactors

  • Increased buffering capacity maintains optimum conditions for protein and antibody production.
  • No detergents or other cell membrane stabilizing agents that can interfere with assays or precipitations.
  • Composition balanced for high-density culture.
WHAT IS IN CDM-HD?
WHAT IS NOT IN CDM-HD?
Customized ratios of amino acids and metabolites Protein
Vitamins and co-factors Hydrolysates
Trace elements Undefined reagents
Micro-nutrients Animal-derived components
Extra buffering capacity Steroid hormones or peptide mitogens
Recombinant growth factors Phenol red
Carbohydrates, including 800 mg of glucose per liter final medium Plurionic Detergent
All low molecular weight components, nothing larger than 3 kD High molecular weight materials including synthetic polymers

cdmhdserum_replacement

3 main benefits of CDM-HD serum replacement

  • 1. A universal serum substitute designed specifically for cells grown at high densities in hollow fiber bioreactors. It is the first time that a cell culture medium is simplified and optimized for a specific bioreactor type.
  • 2. Switching to serum free alternatives such as CDM-HD is promoted by regulatory authorities, industry, and academics for biomedical research and recombinant production purposes, because of undefined composition, risk of contaminations, cost factors and also animal welfare considerations.
  • 3. A chemically defined, animal component free, protein free medium does not contain any proteins nor any materials derived from animals. Purification of the desired secreted product is facilitated, yields can be improved and regulatory compliance is made much simpler. No adaptation is required once the cells have reached high density in the bioreactor.

Serum Starvation vs. 10% CDM-HD

Harvests were taken from confluent T175 flasks with roughly 2.5×106 total cells in each flask. Serum starved flasks were grown up in 10% FBS+DMEM and serum starved for 48 hours prior to collection. CDM-HD media flasks were grown up in 10% FBS+DMEM and switched to 10% CDM-HD+DMEM for 48 hours prior to collection. Each flask contained 35 mL of media. Particle concentrations are as follows (particles/mL): MSC-Serum Starved (4.6×108), MSC-CDM-HD (3.7×109), Prostate-Serum Starved (3.0×109, Prostate-CDM-HD (2.9×1010).

Have an HFBR but are not sure how to use the CDM-HD serum replacement?

Watch our instructional video below, or click to view usage instructions and safety data sheet. Alternatively, contact us for a free consultation.

Instructions for use

Reconstitute the contents of one bottle of CDM-HD to make a total volume of 1 liter. Sterile filter, do not autoclave. Add to any basal medium at a concentration of 10%. When using with FiberCell Systems hollow fiber bioreactors add 10% fetal calf serum to the cell innoculum when loading cells. No adaptation is required though you may see a reduction in glucose uptake rate for the first day or two of use. CDM-HD is stable for at least 2 months as a concentrate when stored at 4 degrees and away from light.

33.36 grams per liter.

Notes:

  • CDM-HD will not require any adaptation but is optimized for cells growing at high density. For best results switch to CDM-HD after cells have reached a high density inside the FiberCell Systems hollow fiber bioreactor, typically after one week of culture or so.
  • Low glucose mediums such as RPMI (2.5 g/L) should be avoided if possible.
  • CDM-HD cannot be used in spinner or stirred tank bioreactors unless plurionic F68 is added.
  • CDM-HD is protein free and does not contain any cell attachment factors. When innoculating cells in medium containing CDM-HD add 10% FBS to the cell innoculum only. This will provide the required attachment factors for adherent cells. This is not required for suspension cells though hybridoma cell lines should be treated as adherent cells.
  • CDM-HD is protein free. The secreted protein of interest may be the only significant protein present in your cell culture supernatant. You should re-evaluate your purification protocols as entire steps can sometimes be eliminated, increasing yield. Keep in mind that CDM-HD is protein free and contains no ferritin so the free iron levels will be higher than in standard mediums. Pay attention to any chelating agents that may be part of your buffers or purification protocol.

Please contact us for more information on our CDM-HD Serum Replacement.

Click here for our US list pricing for 2023. We offer tiered discounted pricing for quantity purchases of our products, 10-25 units, 25+ units, and 50+ units. Please enquire for details. We recommend the most appropriate products for your applications so please feel free to email or phone us to discuss your application and provide you with a quote at info@fibercellsystems.com, 301-471-1269, 240-440-2662. We can accept credit cards and purchase orders from accredited institutions.