GM-100 Glucose Monitoring System
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Connecting Two Bottles of Medium to the Cartridge
The larger cartridges, C2018 and C2003 can contain as many cells as 200-400 roller bottles. They can consume two liters of medium per day or more. You can get 2 and 4 liter autoclavable carboys from Nalgene to use as a medium reservoir or you can simply connect two 1 liter bottles of medium in series as shown in the photo. Simply add the appropriate luer connectors to a short piece of silicone tubing, 12” to 18” in length and connect the two bottles together via one of the stainless steel tubes on each bottle. The other tubes, one each, from each bottle, connect to the cartridge.
Here’s A Way to Squeeze a Little Extra Antibody When You are Producing them from Hybridomas in our Hollow Fiber Cartridges
Simply perform your harvest as usual. One of the issues that many researchers have when using our system is that they are afraid to harvest out too many cells. The cartridge can be overgrown if you don’t remove cells. After centrifugation your cell pellet should be between 1-3 mL or more (with the C5011 as much as 5 mL). Simply decant off the supernatant and re-suspend the cell pellet in 2 pellet volumes of fresh medium that has been diluted 10% with sterile distilled water. Leave it overnight at room temperature. The cells will consume the medium and also the hypotonicity will slightly squeeze the cells causing them to release any intracellularly sequestered antibody. This will get you an extra few milligrams every time you harvest.
The Cells “See” the Volume of Media in the Reservoir Bottle
Too few cells in relation to volume of media can result in lag phase. In the hollow fiber cartridge lag phase can be defined by the cells not consuming glucose but still show significant viability via the trypan exclusion assay. Always seed the cartridge with the recommended cell density. If this is not possible, adjust the volume of media down to keep the cells in culture from being too dilute.If the cells do go into lag phase you can either:
- reduce the volume in the reservoir bottle back down to 125 mL
- inoculate more cells
- wait a few days – sometimes the trick with hollow fiber cell culture is to know when to NOT do something.
Good Laboratory Practices are Key to Productivity
To keep contamination at a minimum, clean the inside of the hood weekly, remembering to remove the working surface and clean underneath. Be sure the hood is validated on a regular basis. Never pour media, always pipette. Always wear a lab coat. Wait 60 seconds for the air currents to stabilize inside the incubator. Pull hair back and be sure to never have bare flesh inside the hood. Use plenty of alcohol and wipes but remember, it is not the application of alcohol that sterilizes, it is the evaporation. Good sterile technique will allow production from the cartridge for many months or more. We have produced a monoclonal antibody for over one year of continuous production.
New cell lines should always be quarantined until a mycoplasma screen has shown negative. The presence of mycoplasma contamination can be hard to detect without specific assays. The culture will just appear to be non-productive.