SMALL Cartridge
4300-C2025 0.1µm pore size for the highest exchange rates. Activated fiber for attachment of matrix proteins, cytokines and antibodies. Ideal fiber for endothelial cell and hepatocyte culture. |
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MEDIUM Cartridges
4300-C2008 Low MWCO (5kd@50%) hydrophilic fiber for trapping smaller molecules. Suggested for recombinant proteins between 25kd and 100kd. Appropriate for suspension cell lines including CHO, HeLa and 293. Can support up to 10 9 cells and produce 100µg/mL of recombinant protein in 15mL ECS. |
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4300-C2011 High MWCO (20kd@50%) hydrophilic fiber for trapping larger molecules, hybridoma culture and lymphocyte culture. 20kd MWCO allows TGF beta and TNF alpha to diffuse away while retaining antibodies. Can support up to 10 9 cells and produce 5-50mg of monoclonal antibody every two days. Also used for medium scale adenovirus production at levels of 1- 5 x 10 12 pfu (plaque forming units).
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LARGE Cartridges
4300-C2003 Low MWCO (5kd@50%) hydrophilic fiber for trapping smaller molecules. Suggested for recombinant proteins between 25kd - 100kd. |
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4300-C2018 High MWCO (20kd@50%) hydrophilic fiber for trapping larger molecules, hybridoma and lymphocyte cultures. 20kd MWCO allows TGF beta and TNF alpha to diffuse away while retaining antibodies. Can support up to 10 11 cells and produce 75-150mg of monoclonal antibody every two days. Appropriate for adherent suspension cell lines including CHO, HeLa and 293 cells. Can support up to 10 11 cells and produce 100µg/mL of recombinant protein in 70mL ECS.
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X-LARGE Cartridges
4300-C4005 The C4005 cartridge is intended for use in larger hollow fiber cell culture systems from other manufacturers and does not include a flow path stand or oxygenator tubing. Side ports have 3" of tubing capped with luer fittings, end ports are 3/8" hose barbs. High gross filtration rate and polysulfone fiber are superior to cellulose acetate for recombinant protein production.
4300-C4020 The C4020 cartridge is intended for use in larger hollow fiber cell culture systems from other manufacturers and does not include a flow path stand or oxygenator tubing. Side ports have 3" of tubing capped with luer fittings, end ports are 3/8" hose barbs with tubing and standard luer connectors on the ends. High gross filtration rate and polysulfone fiber are superior to cellulose acetate for recombinant protein and monoclonal antibody production.
Cartridge/Flowpath Specifications
