ENDOTHELIAL CELL CULTURE

The FiberCell® PS+ module (cat#4300-C2025) is especially designed for the culture and study of endothelial cells under flow. The PS+ fiber can easily be coated with extra-cellular matrix proteins (and/or cytokines and antibodies) to permit the attachment of endothelial cells to the interior wall of the fiber. The microprocessor controlled FiberCell® Systems pump can be programmed to produce consistent and defined amounts of shear stress by regulating the flow

Bovine Aoritc endothelial cells
grown on a matrix of fibronectin.
of medium over the cells. This allows the study of endothelial cells in a more physiologic environment when compared to other methods. As a result, typical cell morphology is retained, a monolayer is formed and tight junctions are created. These culture conditions more closely mimic in vivo cell growth.The microporous nature of the fibers and the ability to control the extra-cellular matrix provides an ideal system for cellular co-cultivation with other cell types such as vascular smooth muscle or neuroglia.
  • Inner surface area of the fibers is equal to a T75 flask
  • Shear stress of .5 dynes/cm2 to 25 dynes/cm2 can be generated
  • Approximately 100 micrograms of RNA can be isolated from each cartridge
  • Most types of microscopy can be performed including immunohisto-chemical techniques

  

SUGGESTED ORDERING FOR ENDOTHELIAL CELL CULTURE:

BIOREACTOR
P3202
The FiberCell® Systems Duet more info
CARTRIDGES
Catalog No.
Size
Surface Area
Fiber Type
Packing Density
ECS Vol
MWCO 50%
Max. Cell#
  
C2025
Small
75 cm2
activated PS
30%
2.5 ml
0.1 µm
108
more info
RESERVOIR CAPS
A1005
33 mm Reservoir Cap more info
A1006
38 mm Reservoir Cap more info