ENDOTHELIAL CELL CULTURE

The FiberCell PS+ module (cat#4300-C2025) is especially designed for the culture and study of endothelial cells under flow. The PS+ fiber can easily be coated with extra-cellular matrix proteins (and/or cytokines and antibodies) to permit the attachment of endothelial cells to the interior wall of the fiber. The microprocessor controlled FiberCell Systems pump can be programmed to produce consistent and defined amounts of shear stress by regulating the flow

Bovine Aoritc endothelial cells
grown on a matrix of fibronectin.
of medium over the cells. This allows the study of endothelial cells in a more physiologic environment when compared to other methods. As a result, typical cell morphology is retained, a monolayer is formed and tight junctions are created. These culture conditions more closely mimic in vivo cell growth.The microporous nature of the fibers and the ability to control the extra-cellular matrix provides an ideal system for cellular co-cultivation with other cell types such as vascular smooth muscle or neuroglia.
  • Inner surface area of the fibers is equal to a T75 flask
  • Shear stress of .5 dynes/cm2 to 25 dynes/cm2 can be generated
  • Approximately 100 micrograms of RNA can be isolated from each cartridge
  • Most types of microscopy can be performed including immunohisto-chemical techniques

  

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SUGGESTED ORDERING FOR ENDOTHELIAL CELL CULTURE:

BIOREACTOR
P3202
The FiberCell Systems Duet more info
CARTRIDGES
Stock No.
Size
Surface Area
Fiber Type
Packing Density
ECS Vol
MWCO 50%
Max. Cell#
  
C2025
Small
75cm2
activated PS
30%
2.5mL
0.1µm
108
more info
RESERVOIR CAPS
A1005
33mm Reservoir Cap more info
A1006
38mm Reservoir Cap more info